Project 9 - Brian Luke

Mechanistic understanding of Rtt101/CUL4 function at sites of single ribonucleotide insertions

We aim at understanding the mechanisms that protect the genome from the adverse consequences of incorporating single ribonucleotides during DNA replication. To this end, we will investigate the activities of a conserved ubiquitin ligase, Cul4/Rtt101, at sites of ribonucleotide-induced replication stress, and determine its impact on replication dynamics by a combination of microscopy, chromatin immunoprecipitation and single DNA fibre analysis. We will characterise the DNA polymerase e subunit Dpb2 as a potential substrate of Cul4/Rtt101 in budding yeast and in the long term elucidate the role of human CUL4 in a genetic disease related to the phenomenon, Aicardi-Goutières syndrome.

Contact

Project 9 - Brian Luke

Prof. Dr Brian Luke

Johannes Gutenberg-University Mainz & Institute of Molecular Biology (IMB)
Ackermannweg 4
55128 Mainz

+49-(0)6131-21465
Email

Webpage Luke lab