Project 21 - Julian Stingele

Global-genome repair of DNA-protein crosslinks: lesion detection and pathway choice

This project utilizes new methodologies and genetic tools to answer key open questions on the detection and repair of covalent DNA-protein crosslinks (DPCs). The repair of DPCs involves the proteolytic destruction of the protein adduct by the DPC-specific protease SPRTN or by proteasomal degradation. In order to monitor DPC repair in mammalian cells, we have devised a novel technique to identify DPCs and to track their fate, which we will employ to understand how the SUMO system detects DPCs and what determines replication-independent DPC repair pathway choice. Ultimately, we will build on these insights to investigate the nature of the endogenous substrates of DPC repair pathways.


Project 21 - Julian Stingele

Prof. Dr Julian Stingele

Gene Center and Department of Biochemistry
Ludwig-Maximilians-Universität München
Postal Address: Feodor-Lynen-Straße 25
Visiting Address: BioSysM, Butenandtstraße 1
81377 Munich


Webpage Stingele lab
Publications since 2023