Project 18 - Karl-Peter Hopfner

Structural mechanism of MRN-ATM in DSB signalling and processing

The MRN complex is a multifunctional nuclease that initiates DNA double-strand break repair, but also contributes to the activation of ATM-dependent damage signalling. Insight into the mechanism of how MRN and ATM interact with each other on DNA, however, is still lacking. Here we address this central question through a structural analysis of complexes of MRN and ATM with DNA, using cryo-electron microscopy. We will also interrogate and complement the structure-derived mechanisms with crosslinking mass spectrometry, biochemistry and collaborative cell-based studies in order to reveal how MRN and ATM cooperate at DNA ends.

Contact

Project 18 - Karl-Peter Hopfner

Prof. Dr Karl-Peter Hopfner

Ludwig-Maximilians-University Munich
Gene Center and Department of Biochemistry
Feodor-Lynen-Straße 25
81377 Munich

+49-(0)89-218076950
Email

Webpage Hopfner lab