This project aims at elucidating how the DNA replication machinery reacts to DNA lesions. We have generated a large collection of engineered human and mouse cells with labeled components of the replication machinery as well as sensors for ubiquitin modifications in live cells. Using these models and a combination of microscopical and genomics approaches, we will generate functional and kinetic spatio-temporal models of the response of the genome replication machinery to damages and how cellular physiology is affected.
Prof. Dr M. Cristina Cardoso
Technische Universität Darmstadt
Webpage Cardoso lab
Publications since 2019
Project summary first funding period (2019-2022)