In this project, we investigate the mechanism of homologous recombination (HR) with a particular focus on two novel HR factors, RAD54B and RECQ1. Recent work from us and others has delineated two sub-pathways of HR which have distinct outcomes and involve specific genetic elements. We propose that both RAD54B and RECQ1 are specific factors for one of the two sub-pathways. The project further aims to screen for additional factors specific for one of the two HR sub-pathways and to establish assays that allow the investigation of a fundamental HR intermediate, the so-called D-loop.
Roles of helicases and recombinases in homologous recombination pathway choice