Project summary 2019-2022

Cristina Cardoso: Dissecting the response of replisomes to genotoxic stress (Project 6, 2019-2022)

Our objective is to understand how cells respond to genotoxic insults during replication of their genome. We will fluorescently label selected replication factors and use superresolution microscopy, live-cell imaging and DNA combing to monitor the dynamics of replisomes in different chromatin environments and to measure their responses to agents that block polymerases or damage the replication template. In this manner, we aim to assemble functional and kinetic/spatiotemporal models to explain the dynamic interplay between DNA replication and repair.